Application

Research CategoryApoptosis & Cancer

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected FANCJ/BACH1 in MCF-7 cells (Cantor, S. B., et al. (2001). Cell. 105(1):149-160.).

Western Blotting Analysis: A representative lot from an independent laboratory detected FANCJ/BACH1 in transfected FA-J cell lysate (Xie, J., et al. (2010). Oncogene. 29(17):2499-2508.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated FANCJ/BACH1 from MCF-7 cell lysate, prior to detecting the immunoprecipitated sample in Western Blot (Cantor, S. B., et al. (2001). Cell. 105(1):149-160.).

Research Sub CategoryApoptosis - Additional

This Anti-FANCJ/BACH1 Antibody, clone 2G7 is validated for use in western blotting, ICC & IP for the detection of FANCJ/BACH1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bach1 and Bach2 constitute a subfamily of the basic region-leucine zipper family. Bach proteins form heterodimers with small Maf proteins, bind to the Maf recognition element (MARE) and repress target gene expression. Transcription regulator protein BACH2 mediates early B cell differentiation and is specifically expressed in pro and mature B-cells. This protein plays an important role in both the immunoglobulin class switch, oncogene-induced senescence, and somatic hypermutation.

Immunogen

Linear peptide corresponding to the C-terminus of human FANCJ/BACH1.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected FANCJ/BACH1 in 10 µg of MCF-7 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~140 kDa observed